| Identification | Back Directory | [Name]
Ingenol-3-angelate | [CAS]
75567-37-2 | [Synonyms]
Picato
Ingenol mebutate
Ingenol 3-mebutate
Ingenol-3-O-angelate
Ingenol Mebutate, >98%
Ingenol Mebutate (PEP005)
Ingenol mebutate USP/EP/BP
PEP005; INGENOL MEBUTATE; I3A
Ingenol-3-O-angelate 75567-37-2
Ingenol-3-angelate
Ingenol Mebutate
(1aR,2S,5R,5aS,6S,8aS,9R,10aR)-5,5a-Dihydroxy-4-(hydroxymethyl)-1,1,7,9-tetramethyl-11-oxo-1a,2,5,5a,6,9,10,10a-octahydro-1H-2,8a- methanocyclopenta(a)cyclopropa(e)cyclodecen-6-yl (2Z)-2-methylbut-2-enoate
(2Z)-2-Methyl-2-butenoic acid (1aR,2S,5R,5aS,6S,8aS,9R,10aR)-1a,2,5,5a,6,9,10,10a-octahydro-5,5a-dihydroxy-4-(hydroxymethyl)-1,1,7,9-tetramethyl-11-oxo-1H-2,8a-methanocyclopenta[a]cyclopropa[e]cyclodecen-6-yl ester | [Molecular Formula]
C25H34O6 | [MDL Number]
MFCD07784504 | [MOL File]
75567-37-2.mol | [Molecular Weight]
430.534 |
| Chemical Properties | Back Directory | [Boiling point ]
576.9±50.0 °C(Predicted) | [density ]
1.26±0.1 g/cm3(Predicted) | [storage temp. ]
?20°C | [solubility ]
DMSO: soluble15mg/mL, clear | [form ]
powder | [pka]
11.52±0.70(Predicted) | [color ]
white to beige | [Water Solubility ]
Soluble in 100% ethanol, DMSO, dichloromethane, and methanol. Insoluble in water. | [Stability:]
Stable for 1 year as supplied. Solutions in DMSO or ethanol may be stored at -20°C for up to 1 month. |
| Questions And Answer | Back Directory | [Ingenol mebutate]
Ingenol mebutate (PicatoTM, Leo Pharma), a macrocyclic diterpene ester, is the active constituent in the sap of Euphorbia peplus. Commonly known as radium weed, petty spurge, or milkweed, E. peplus has been used for centuries as a traditional remedy for cutaneous conditions including skin cancer.
Owing to a substantially shorter course of treatment (2-3 days) and duration of local cutaneous reaction, ingenol mebutate offers an appealing alternative to other topical agents used for the treatment of actinic keratoses.
| [Mechanism of action]
Ingenol mebutate has a dual mechanism of action. Ingenol mebutate first induces rapid and direct cell death within hours of application followed by an acute inflammatory response that eliminates residual tumor cells.
In vitro and in vivo studies on B16 mouse melanoma cells exposed to ingenol mebutate revealed disruption of the cell membrane, loss of mitochondrial membrane potential, and mitochondrial swelling resulting in rapid primary necrosis. As a result of the rapid destruction of tumor cells, a treatment duration of 2–3 days is sufficient. Unlike other anti-cancer agents, ingenol mebutate does not trigger apoptosis, and thus the development of apoptosis resistance in tumor cells is unlikely to compromise its activity.
Following the initial cell necrosis, an acute inflammatory response occurs. Ingenol mebutate activates protein kinase c resulting in the release of pro-inflammatory cyto-kines (IL-1B, IL-8, TNF-alpha), the production of tumor-specific antibodies, an enhanced endothelial adhesion molecule expression, and a substantial infiltration of neutro-phils which generate tumoricidal reactive oxygen intermediates. The end-result is eradication of residual tumor cells via neutrophil mediated antibody depen-dent cellular cytotoxicity (ADCC). The elimination of resid-ual tumor cells is thought to be essential in preventing relapse. Furthermore, the inflammatory response appears to confer a favorable cosmetic outcome via expedited healing and a rapid regeneration of the normal cutaneous architecture.
| [Clinical Use]
Ingenol mebutate is the active ingredient in the sap from the Euphorbia peplus plant that has been used to treat a variety of skin lesions including warts, AKs, and NMSC. Recent clinical trials have demonstrated that two or three applica-tions of ingenol mebutate is effective in clearing AKs and BCCs. The efficacy of the treatment increased in a dose dependent manner with only mild dose dependent dermatological side effects, confirming that short term use of ingenol mebutate is safe when treating a variety of skin lesions. | [Dosage and Administration]
Ingenol mebutate (chemical structure C25H34O6) has been formulated as a topical gel. Each gram contains 150 or 500 mcg of ingenol mebutate in a base of isopropyl alcohol, hydroxyethyl cellulose, citric acid monohydrate, sodium citrate, benzyl alcohol, and purified water.
For the indication of actinic keratoses. The 0.015 % gel is applied once daily for three consecutive days (face or scalp) while the 0.05 % gel is applied once daily for two consecu-tive days (trunk or extremities).
| [side effects]
The most common side effects of ingenol mebutate were application-site reactions including erythema, scale, crust-ing, edema, vesiculation, erosions, ulceration, pruritus, pain, and irritation. Skin reactions typically occurred within 1 day of treatment initiation, peaking in intensity 1 week following application, and resolving within 2 weeks on the face/scalp and 4 weeks on the trunk/extremities.
Reports of periocular pain, edema, ptosis have been docu-mented. Prompt hand washing after application and avoid-ance of contact with the eyes is recommended.
No evidence of skin sensitization, photoirritation, or pho-toallergic potential has been demonstrated.
Ingenol mebutate is pregnancy category C. It is unknown if ingenol mebutate is secreted in the breast milk of lactating women.
|
| Hazard Information | Back Directory | [Description]
Ingenol mebutate (also known as PEP005) was approved in January 2012
by the US FDA for the topical treatment of actinic keratoses (AK). Ingenol mebutate also received approval in 2012 in the European Union, Australia,
and Brazil for the same indication. Ingenol
mebutate, a diterpene ester natural product, is the active agent in the sap
of the plant Euphorbia peplus, which has long been used as a traditional remedy for skin lesions. Ingenol mebutate has a novel mechanism of action
involving initial plasma membrane disruption, rapid loss of mitochondrial
membrane potential, and cell death by primary necrosis within 1 h;
a subsequent tumor-specific immune response results in antibodydependent
cellular toxicity that eliminates residual cells. Ingenol mebutate
is obtained by extraction from the dried, milled aerial parts of Euphorbia peplus followed by a series of purification steps. | [Originator]
Peplin (United States) | [Uses]
Ingenol 3-Angelate is known to exhibit antitumor activities which induces plasma membrane and mitochondrial disruption and necrotic cell death. | [Definition]
ChEBI: A tetracyclic diterpenoid ester obtained by formal condensation of the carboxy group of (2Z)-2-methylbut-2-enoic (angelic) acid with the 3-hydroxy group of ingenol. Used for the topical treatment of actinic keratosis. | [Brand name]
Picato | [Biochem/physiol Actions]
Ingenol-3-angelate, is a phorbol ester-like compound or a diacylglycerol analog that is used in the treatment of several disorders like skin cancer. It is also known as PEP005. Ingenol-3-angelate stimulates the initiation of the enzyme by providing PKCs (protein kinase C) to cellular membranes. | [Synthesis]
Although
several synthetic approaches to the ingenol family of terpenes have been reported,97-113 Liang and
coworkers at LEO Pharma have reported a semisynthesis of the API from naturally-occurring ingenol.
This natural product?ˉs accessibility from the seeds of E. lathyris renders it widely commercial on scale.
The conversion of ingenol to ingenol mebutate involves a protection, esterification, and deprotection
strategy to procure scale quantities of the drug.114 Conversion of ingenol (87) to the
corresponding 5,20-acetonide 88 proceeded in good yield using a protocol modified from the original
conditions described by Hecker. A considerable amount of study was conducted by Liang to affect
efficient angeloylation with minimal isomerization of the double-bond to the corresponding Z-isomer
(tiglate). It was found that angelic anhydride 89 (which is a commercially available reagent, but for
process scale was prepared immediately prior to usage from the self-condensation of 99.5% pure angelic
acid with 0.5 equivalents of DCC) in the presence of LHMDS gave acetonide 90 in over 95%
conversion and was practically free of the undesired tiglate bi-product after recrystallization (73%
yield).96 Deprotection of the acetonide 90 was affected using phosphoric acid and after three
recrystallizations, ingenol mebutate (XIV) was produced on multigram scale in a combined yield of
37% starting from ingenol 87.
| [target]
gp120/CD4 | HIV | PKC | P-gp | NF-kB | ERK | [storage]
-20°C | [References]
References/Citations |
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