3102-57-6
3102-57-6 结构式
基本信息
CERAMIDE C2
C2 CERAMIDE
C2:0 CERAMIDE
TOC Ceramide
ACETYL CERAMIDE
C14 SPHINGOID BASE
N-ACETYLSPHINGOSINE
C2-D-ERYTHRO-CERAMIDE
N-ACETYL-D-SPHINGOSINE
物理化学性质
常见问题列表
Protein phosphatase 1
PP2A
Ceramide-activated protein phosphatase (CAPP)
Apoptosis
Mitochondrial respiratory chain complex III
C2 Ceramide (5 nM-200 µM; 24 hours; primary mouse osteoblasts) treatment (≤500 nM) promots osteoblast viability, whilst concentrations ≥2 µM significantly reduces osteoblast viability in a dose- and time-dependent manner.
C2 Ceramide increases cytoplasmic histone-associated DNA fragments by 5.7- and 11.2-fold at 50 µM and 100 µM C2 Ceramide concentrations respectively in osteoblasts. At these higher concentrations, C2 Ceramide is a potent inducer of apoptosis in osteoblasts.
C2 Ceramide up-regulates mRNA expression of angiogenic genes in human dental pulp cells (HDPCs) and increases the migration and capillary tube formation of endothelial cells, whereas PP1 small interfering RNA shows opposite effects. Human dental pulp cells (HDPCs) increases levels of bone morphogenetic protein 2, phosphorylation of Smad 1/5/8, and mRNA expression of runt-related transcription factor 2 and osterix.
Cell Viability Assay
| Cell Line: | Primary mouse osteoblasts |
| Concentration: | 5 nM-200 µM |
| Incubation Time: | 24 hours |
| Result: | Murine osteoblasts demonstrated a dose-dependent increase in their survival rate when exposed to low concentrations of 5-500 n M. Increasing concentrations of 20-200µM caused a dose-dependent decrease in mitochondrial succinate dehydrogenase activity and osteoblast survival. |
The PP1 activator C2 Ceramide increases alkaline phosphatase activity, mineralizes nodule formation, and mRNA expression of dentin matrix protein 1 and dentin sialophosphoprotein. In contrast, knockdown by PP1 small interfering RNA inhibits odontoblastic differentiation.