52589-11-4

中文名称黄柏苷

英文名称 phellamurin

CAS 52589-11-4

分子式 C26H30O11

分子量 518.52

MOL 文件 52589-11-4.mol

更新日期 2024/03/08 11:56:32

52589-11-4 结构式

基本信息物理化学性质常见问题列表

基本信息

中文别名

黄柏苷
黄柏甙
二氢黄柏苷
(二氢)黄柏甙

英文别名

phellamurin
(dihydro) Phellodendron
(2R)-7-(β-D-Glucopyranosyloxy)-2,3-dihydro-3β,5-dihydroxy-2α-(4-hydroxyphenyl)-8-(3-methyl-2-butenyl)-4H-1-benzopyran-4-one
4H-1-Benzopyran-4-one,7-(b-D-glucopyranosyloxy)-2,3-dihydro-3,5-dihydroxy-2-(4-hydroxyphenyl)-8-(3-methyl-2-buten-1-yl)-,(2R,3R)-
4H-1-Benzopyran-4-one, 7-(β-D-glucopyranosyloxy)-2,3-dihydro-3,5-dihydroxy-2-(4-hydroxyphenyl)-8-(3-methyl-2-buten-1-yl)-, (2R,3R)-

物理化学性质

沸点861.5±65.0 °C(Predicted)

密度1.504±0.06 g/cm3(Predicted)

溶解度Soluble in Chloroform,Dichloromethane,Ethyl Acetate,DMSO,Acetone,etc.

酸度系数(pKa)7.15±0.60(Predicted)

形态粉末

常见问题列表

生物活性

Phellamurin 是一种来自 Phellodendron amurense 叶的植物黄酮糖苷，可抑制肠道 P-糖蛋白 (P-glycoprotein)。Phellamurin 还抑制凤蝶的产卵。Phellamurin 诱导细胞凋亡 (apoptosis)，并具有抗肿瘤活性。

体外研究

Phellamurin (0-10 μg/mL; 48 hours; U2OS and Saos-2 cells) treatment leads to a repression of cell viability in U2OS and Saos-2 cells in a dose-dependent manner.
Phellamurin (0-10 μg/mL; 48 hours; U2OS and Saos-2 cells) treatment concentration-dependently promots the apoptosis of U2OS and Saos-2 cells.
Phellamurin (0-10 μg/mL; 48 hours; U2OS and Saos-2 cells) treatment declines the ratios of p-PI3K/PI3K, p-AKT/AKT, and p-mTOR/mTOR in U2OS and Saos-2 cells.

Cell Viability Assay

Cell Line:	U2OS and Saos-2 cells
Concentration:	0 μg/mL, 2.5 μg/mL, 5 μg/mL, and 10 μg/mL
Incubation Time:	48 hours
Result:	Suppressed the viability of U2OS and Saos-2 cells in a concentration-dependent manner.

Apoptosis Analysis

Cell Line:	U2OS and Saos-2 cells
Concentration:	0 μg/mL, 2.5 μg/mL, 5 μg/mL, and 10 μg/mL
Incubation Time:	48 hours
Result:	Induced apoptosis of U2OS and Saos-2 cells in a concentration-dependent manner.

Western Blot Analysis

Cell Line:	U2OS and Saos-2 cells
Concentration:	0 μg/mL and 10 μg/mL
Incubation Time:	48 hours
Result:	Repressed the PI3K/AKT/mTOR pathway in U2OS and Saos-2 cells.

体内研究

Phellamurin (50 mg/kg/day; intraperitoneal injection; daily; for 21 days; female BALB/c nude mice) treatment represses osteosarcoma tumor growth in vivo. The ratios of p-PI3K/PI3K, p-AKT/AKT, and p-mTOR/mTOR are decreased in xenograft in Phellamurin-treated mice.

Animal Model:	Athymic female BALB/c nude mice (6 weeks old ) injected with U2OS cells
Dosage:	50 mg/kg/day
Administration:	Intraperitoneal injection; daily; for 21 days
Result:	Repressed osteosarcoma tumor growth in vivo.