607737-87-1
中文名称
A 77-01
英文名称
4-[3-(6-METHYL-2-PYRIDINYL)-1H-PYRAZOL-4-YL]-QUINOLINE
CAS
607737-87-1
分子式
C18H14N4
分子量
286.33
MOL 文件
607737-87-1.mol
更新日期
2024/05/14 10:36:02
607737-87-1 结构式
基本信息
中文别名
TGF-Β I型受体家族ALK5抑制剂(A 77-01)4-[3-(6-甲基-2-吡啶基)-1H-吡唑-4-基]喹啉
英文别名
A77-01A-77-01
A 77-01
CS-1775
A7701
A 7701
A-7701
4-[3-(6-Methylpyridin-2-yl)-1H-pyrazol-4-yl]quinoline
4-[3-(6-METHYL-2-PYRIDINYL)-1H-PYRAZOL-4-YL]-QUINOLINE
Quinoline, 4-[3-(6-methyl-2-pyridinyl)-1H-pyrazol-4-yl]-
4-[5-(6-methylpyridin-2-yl)-1~{H}-pyrazol-4-yl]quinoline
4-[3-(6-Methyl-2-pyridinyl)-1H-pyrazol-4-yl]quinoline A 77-01
所属类别
生物化工：激动剂抑制剂物理化学性质
储存条件-20°C储存
溶解度insoluble in H2O; ≥14.3 mg/mL in DMSO; ≥8.84 mg/mL in EtOH with gentle warming and ultrasonic
形态固体
常见问题列表
生物活性
A 77-01 是一种有效的 TGF-β I 型受体家族 ALK5 抑制剂，IC50 值为 25 nM。靶点
IC50: 25 nM (ALK5)
体外研究
A-77-01 (0.01-10 µM; 25 hours; Mv1Lu cells) treatment effectively inhibits TGF-β-induced transcriptional activation in concentration-dependent fashion.
A-77-01 (1 µM; 24-72 hours; Mv1Lu cells) treatment efficiently prevents the growth-inhibitory effects of TGF-β.
A-77-01 (1 µM; 30 minutes; HaCaT cells) treatment inhibits TGF-β-induced phosphorylation of Smad2.
RT-PCR
| Cell Line: | Mv1Lu cells |
| Concentration: | 0.01 µM, 0.03 µM, 0.1 µM, 0.3 µM, 1 µM, 3 µM, 10 µM |
| Incubation Time: | 25 hours |
| Result: | Inhibited the luciferase activity induced by TGF-β in concentration-dependent fashion. |
Cell Viability Assay
| Cell Line: | Mv1Lu cells |
| Concentration: | 1 µM |
| Incubation Time: | 24 hours, 48 hours, 72 hours |
| Result: | Efficiently prevented the growth-inhibitory effects of TGF-β. |
Western Blot Analysis
| Cell Line: | HaCaT cells |
| Concentration: | 1 µM |
| Incubation Time: | 30 minutes |
| Result: | Inhibited TGF-β-induced phosphorylation of Smad2. |