CU-T12-9
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- CAS号:
- 1821387-73-8
- 英文名:
- CU-T12-9
- 英文别名:
- CU-T12-9;N-methyl-4-nitro-2-[4-[4-(trifluoromethyl)phenyl]imidazol-1-yl]aniline;Benzenamine, N-methyl-4-nitro-2-[4-[4-(trifluoromethyl)phenyl]-1H-imidazol-1-yl]-;obesity,inflammatory,carcinoma,cancer,Toll-like Receptor (TLR),TLR1/2,diseases,specific,infectious,bladder,CU-T-12-9,CUT129,Inhibitor,breast,asthma,CU T12 9,inhibit,influenza,pancreatic
- 中文名:
- CU-T12-9
- 中文别名:
- 化合物CU-T12-9;CU-T12-9,TLR1 / 2激动剂;N-甲基-4-硝基-2-(4-(4-(三氟甲基)苯基)-1H-咪唑-1-基)苯胺
- CBNumber:
- CB03146280
- 分子式:
- C17H13F3N4O2
- 分子量:
- 362.31
- MOL File:
- 1821387-73-8.mol
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CU-T12-9化学性质
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沸点:
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524.3±50.0 °C(Predicted)
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密度:
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1.39±0.1 g/cm3(Predicted)
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储存条件:
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Store at -20°C
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溶解度:
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DMSO: 250 mg/mL (690.02 mM)
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形态:
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A solid
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酸度系数(pKa):
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3.53±0.10(Predicted)
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CU-T12-9性质、用途与生产工艺
CU-T12-9 是一种特异性 TLR1/2 激动剂，EC50 为 52.9 nM。CU-T12-9 既激活先天免疫系统，又激活适应性免疫系统。CU-T12-9 选择性激活 TLR1/2 异二聚体而对 TLR2/6 没有作用。CU-T12-9 通过促进 TLR1 和 TLR2 二聚而激活 NF-κB 信号，致使下游 TNF-α，IL-10，和 iNOS 增加，从而提高身体的免疫反应。
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TLR2
52.9 nM (EC
50
, in HEK-Blue cells)
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TLR1
52.9 nM (EC
50
, in HEK-Blue cells)
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CU-T12-9 directly targets TLR1/2 to initiate downstream signaling. By binding to both TLR1 and TLR2, CU-T12-9 facilitates the TLR1/2 heterodimeric complex formation, which in turn activates the downstream signaling.
CU-T12-9 activates the TLR1/2 pathway by inducing NF-κB activation to trigger downstream signaling, such as secreted embryonic alkaline phosphatase (SEAP), NO, and TNF-α.
CU-T12-9 (0.39-100 μM; 24 hours) does not produce toxicity up to 100 μM in HEK-Blue hTLR2 and Raw 264.7 cells.
CU-T12-9 up-regulates the mRNA levels of TLR1, TLR2, TNF, IL-10, and iNOS. CU-T12-9 (0.1-10 μM) activates TLR1 mRNA and iNOS mRNA after Raw 264.7 cells are treated for 24 hours. CU-T12-9 (0.1-10 μM) activates TLR2 and IL-10 mRNA after Raw 264.7 cells are treated for 2 hours. CU-T12-9 (0.1-10 μM) activates TNF mRNA after Raw 264.7 cells are treated for 8 hours.
Cell Cytotoxicity Assay
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Cell Line:
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HEK-Blue hTLR2 and Raw 264.7 macrophage cells
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Concentration:
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0.39, 0.78, 1.56, 3.125, 6.25, 12.5, 25, 50, and 100 μM
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Incubation Time:
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24 hours
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Result:
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No toxicity was seen up to 100 μM.
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RT-PCR
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Cell Line:
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Raw 264.7 cells
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Concentration:
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0.1, 1, 10 μM
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Incubation Time:
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24 hours for TLR1 and iNOS mRNA assay
2 hours for TLR2 and IL-10 mRNA assay
8 hours for TNF mRNA assay
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Result:
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Triggered TLR1 mRNA and iNOS mRNA at 24 hours dose-dependently.
Dose-dependent activation of TLR2 mRNA and IL-10 mRNA at 2 hours.
Showed dose-dependent activation of TNF mRNA at 8 hours.
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CU-T12-9
上下游产品信息
上游原料
下游产品
| 更新日期 | 产品编号 | 产品名称 | CAS编号 | 包装 | 价格 |
|---|
| 2024/04/30 | HY-110353 | CU-T12-9 | | 1 mg | 522元 |
| 2024/04/30 | HY-110353 | CU-T12-9
CU-T12-9 | 1821387-73-8 | 5mg | 1100元 |
1821387-73-8, CU-T12-9 相关搜索:
- 药靶配体
- CU-T12-9,TLR1 / 2激动剂
- N-甲基-4-硝基-2-(4-(4-(三氟甲基)苯基)-1H-咪唑-1-基)苯胺
- 化合物CU-T12-9
- 1821387-73-8
- obesity,inflammatory,carcinoma,cancer,Toll-like Receptor (TLR),TLR1/2,diseases,specific,infectious,bladder,CU-T-12-9,CUT129,Inhibitor,breast,asthma,CU T12 9,inhibit,influenza,pancreatic
- Benzenamine, N-methyl-4-nitro-2-[4-[4-(trifluoromethyl)phenyl]-1H-imidazol-1-yl]-
- N-methyl-4-nitro-2-[4-[4-(trifluoromethyl)phenyl]imidazol-1-yl]aniline
- CU-T12-9