石松鹼
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- CAS号:
- 466-61-5
- 英文名:
- lycopodine
- 英文别名:
- lycopodine;(15R)-15-Methyllycopodan-5-one;1,9-Ethanobenzo[i]quinolizin-14-one, dodecahydro-11-methyl-, (1S,8aR,9S,11R,12aR)-
- 中文名:
- 石松鹼
- 中文别名:
- 石松鹼;化合物 T13764
- CBNumber:
- CB41480614
- 分子式:
- C16H25NO
- 分子量:
- 247.38
- MOL File:
- 466-61-5.mol
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石松鹼化学性质
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熔点:
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114-115°; mp (racemate) 130-131°
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比旋光度:
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D -24° (alc)
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沸点:
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125 °C(Press: 0.2 Torr)
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密度:
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1.18 g/cm3
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储存条件:
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Store at -20°C
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溶解度:
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Soluble in DMSO
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酸度系数(pKa):
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9.36±0.40(Predicted)
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石松鹼性质、用途与生产工艺
Lycopodine，一种石松 (Lycopodium clavatumspores) 孢子重要的生物活性成分，通过调节难治性前列腺癌细胞中 5-脂氧合酶 (5-lipoxygenase)，极化线粒体膜电位而不调节 p53 活性来诱导细胞凋亡。 Lycopodine 通过激活 caspase-3 诱导细胞凋亡来抑制 HeLa 细胞的增殖。
Lycopodine (5.22-78.3 μg/mL; 12 hours) has 50% viability at 57.62±0.086 μg/mL and 51.46±1.43 μg/mL for PC3 and LnCaP, respectively.
Treated with Lycopodine (74-222 mM; 12 hours), the apoptotic index is with respect to the gradual increase in doses for the PC3 and LnCaP cells.
Lycopodine (74-222 mM; 12 hours) induces cell cycle arrest at G0/G1 phase in PC3 and LnCaP cells.
Lycopodine (0-200 µg/mL; 48 hours) shows cytotoxicity to HeLa cells in a dose and time dependent manner. However, Lycopodine shows minimal cytotoxic effects in normal peripheral blood mononuclear cells (PBMC) even at the highest dose (200 µg/mL).
Lycopodine (100, 200 µg/mL; 24 hours) increases level of Bax and decreases the mitochondrial cytochrome c. This is followed by an increase in expression of cytochrome c in cytosolic fraction. Lycopodine also cleaves the caspase-3 in the total cell lysate, while the expression of Bcl-2 is down regulated.
Cell Viability Assay
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Cell Line:
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PC3 and LnCaP cells
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Concentration:
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5.22-78.3 μg/mL
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Incubation Time:
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12 hours
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Result:
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Reached 50% viability at 57.62±0.086 μg/mL and 51.46±1.43 μg/mL for PC3 and LnCaP, respectively.
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Apoptosis Analysis
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Cell Line:
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PC3 and LnCaP cells
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Concentration:
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74, 148, 222 mM
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Incubation Time:
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12 hours
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Result:
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The apoptotic index was with respect to the gradual increase in doses.
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Cell Cycle Analysis
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Cell Line:
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PC3 and LnCaP cells
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Concentration:
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74, 148, 222 mM
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Incubation Time:
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12 hours
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Result:
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Arrested growth of the cells at G0/G1 phase in the case of PC3 and LnCaP cells.
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Cell Cytotoxicity Assay
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Cell Line:
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Hela cells and PBMC
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Concentration:
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0-200 µg/mL
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Incubation Time:
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48 hours
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Result:
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A linear increase of the cytotoxicity was along with the increase of time of treatment and also of the dose.
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Western Blot Analysis
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Cell Line:
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HeLa cells
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Concentration:
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100, 200 µg/mL
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Incubation Time:
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24 hours
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Result:
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Increased level of Bax and decreased the mitochondrial cytochrome c.
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石松鹼
上下游产品信息
上游原料
下游产品
466-61-5, 石松鹼 相关搜索:
- 抑制剂
- 化合物 T13764
- 石松鹼
- 466-61-5
- 1,9-Ethanobenzo[i]quinolizin-14-one, dodecahydro-11-methyl-, (1S,8aR,9S,11R,12aR)-
- (15R)-15-Methyllycopodan-5-one
- lycopodine