Geneticin

Geneticin Properties

storage temp.	2-8°C
solubility	H₂O: 50 mg/mL
form	white powder
color	white to off-white
optical activity	[α]/D (Specific Rotation: +104.4o (c=0.3% in H2O at 26oC))
Water Solubility	soluble
Stability	Hygroscopic
InChIKey	QNQZPJLBGRQFDD-ZLRXUJDMSA-N
FDA UNII	A08F5XTI6G

SAFETY

Risk and Safety Statements

Symbol(GHS)	GHS08
Signal word	Danger
Hazard statements	H317-H334
Precautionary statements	P261-P272-P280-P284-P302+P352-P333+P313
Hazard Codes	Xn,Xi
Risk Statements	20/21/22-36/37/38-42/43
Safety Statements	22-24/25-36/37-23-36-26-45
WGK Germany	3
RTECS	CB9378500
HS Code	29419090

Geneticin price More Price(60)

Manufacturer	Product number	Product description	CAS number	Packaging	Price	Updated	Buy
Sigma-Aldrich	G418-RO	G-418 Solution	108321-42-2	20ml	$183	2024-03-01	Buy
Sigma-Aldrich	G418-RO	G-418 Solution solution, =98% (TLC), suitable for transfection	108321-42-2	100X100ML	$747	2022-05-15	Buy
Sigma-Aldrich	5.09290	OmniPur G418 Sulfate, Cell Culture Tested - CAS 108321-42-2 - Calbiochem	108321-42-2	500mg	$113	2022-05-15	Buy
Sigma-Aldrich	5.09290	OmniPur G418 Sulfate, Cell Culture Tested - CAS 108321-42-2 - Calbiochem	108321-42-2	1g	$145	2022-05-15	Buy
Sigma-Aldrich	5.09227	G 418 Sulfate, Sterile-Filtered Solution in PBS, Cell Culture-Tested - CAS 108321-42-2 - Calbiochem	108321-42-2	20ml	$124	2022-05-15	Buy

Product number	Packaging	Price	Buy
G418-RO	20ml	$183	Buy
G418-RO	100X100ML	$747	Buy
5.09290	500mg	$113	Buy
5.09290	1g	$145	Buy
5.09227	20ml	$124	Buy

Geneticin Chemical Properties,Uses,Production

Description

Geneticin (also known as G418, G418 sulfate) is an aminoglycoside antibiotic similar in structure to gentamicin B1, produced by Micromonospora rhodorangea. Geneticin blocks polypeptide synthesis by inhibiting the elongation step in both prokaryotic and eukaryotic cells and is commonly used in laboratory research to select genetically engineered cells. Resistance to Geneticin is conferred by the neo gene from Tn5 encoding an aminoglycoside 3'-phosphotransferase, APH 3'II.

Chemical Properties

white to off-white powder

Uses

Geneticin is an aminoglycoside antibiotic similar in structure to gentamicin used for selection and maintenance of prokaryotic and eukaryotic cells. Optimal concentrations for selection and maintenance vary by cell lines. Bacteria and algae require 5 μg/mL or less while animal cells may require 300-500 μg/mL. It is also used to membrane-permeant allosteric effector of hemoglobin therapeutic in cardiovascular diseases and cancer.

Application

Geneticin's most common application is in molecular biology as a selection agent. Geneticin(G418 sulfate) is toxic to bacteria, yeast, protozoa, helminths, and mammalian cells. Resistance is conferred by one of two dominant genes of bacterial origin which can be expressed in eukaryotic cells. G418 is water soluble and can be stored at room temperature for as long as 1 year. Aqueous solutions should be stored frozen. The amount of G418 required for selection will vary with each cell type and growth cycle. Although cells that are multiplying will be affected sooner than those that are not, cells that are in log phase will still require 3 to 7 days for selection. In general, the amount of G418 required for selection of mammalian cells is 400 mg/ml and 200 mg/ml for maintenance.

General Description

The optimal concentration of G418 for selection of resistance will vary according to the organism and/or cell type under investigation. In general, the concentration of active drug required for selection is as follows: Dictyostelium sp.: 10-100 μg/ml Plant cells: 10-100 μg/ml Yeast cells: 0.5-1.0 μg/ml Mammalian cells: 0.1-2.0 μg/ml

Biochem/physiol Actions

Mode of Action: G418 blocks polypeptide synthesis and protein elongation by inhibiting synthesis at the 70S and 80S ribosomes.Antimicrobial Spectrum: G418 selects for cells stably transfected with an iNOS promoter construct and neomycin resistance gene and shows activity against protozoa and helminthes.

storage

+4°C

Mode of action

Geneticin works by blocking polypeptide synthesis by preventing the elongation step within eukaryotic cells.
Resistance: Resistance to Geneticin is conferred through the NPTll gene in plant cells and the NEO gene in mammalian cells, which encodes aminoglycoside 3'-phosphotransferase.
Action: Geneticin functions by causing a chain of nonsense mutations during translation, ultimately resulting in inhibition of polypeptide synthesis and protein elongation.

References

[1] bar-nun s, shneyour y, beckmann js. g-418, an elongation inhibitor of 80 s ribosomes. biochim biophys acta. 1983, 741 (1): 123-7.
[2] julian davies and antonio jimenez. a new selective agent for eukaryotic cloning vectors. am. j. trop. med. hyg. 1980, 29 (5): 1089-1092.
[3] xianchao g. zhang, peter w. mason, edward j. dubovi, xiaodong xu, nigel bourne, randall w. renshaw, timothy m. block, and alexander v. birk. antiviral activity of geneticin against dengue virus. antiviral res. 2009 july, 83(1): 21–27.

Geneticin Preparation Products And Raw materials

Raw materials

Preparation Products

Geneticin Suppliers

Global( 424)Suppliers

Supplier	Tel	Email	Country	ProdList	Advantage
Hubei Ipure Biology Co., Ltd	+8613367258412	ada@ipurechemical.com	China	10326	58
Hebei Mojin Biotechnology Co., Ltd	+8613288715578	sales@hbmojin.com	China	12471	58
Firsky International Trade (Wuhan) Co., Ltd	+8615387054039	admin@firsky-cn.com	China	436	58
Henan Tianfu Chemical Co.,Ltd.	+86-0371-55170693 +86-19937530512	info@tianfuchem.com	China	21689	55
Hangzhou FandaChem Co.,Ltd.	008657128800458; +8615858145714	fandachem@gmail.com	China	9341	55
career henan chemical co	+86-0371-86658258	sales@coreychem.com	China	29914	58
TianYuan Pharmaceutical CO.,LTD	+86-755-23284190 13684996853	sales@tianpharm.com	CHINA	304	58
Hubei Jusheng Technology Co.,Ltd.	18871490254	linda@hubeijusheng.com	CHINA	28180	58
Yunbio Tech Co.,Ltd.	+86-010-60605551 +86-18046518538	yunbiochem@126.com	China	321	58
Hebei Guanlang Biotechnology Co., Ltd.	+86-19930503282	alice@crovellbio.com	China	8822	58

Supplier	Advantage
Hubei Ipure Biology Co., Ltd	58
Hebei Mojin Biotechnology Co., Ltd	58
Firsky International Trade (Wuhan) Co., Ltd	58
Henan Tianfu Chemical Co.,Ltd.	55
Hangzhou FandaChem Co.,Ltd.	55
career henan chemical co	58
TianYuan Pharmaceutical CO.,LTD	58
Hubei Jusheng Technology Co.,Ltd.	58
Yunbio Tech Co.,Ltd.	58
Hebei Guanlang Biotechnology Co., Ltd.	58

View Lastest Price from Geneticin manufacturers

Update time	Product	Price	Min. Order	Purity	Supply Ability	Manufacturer
2023-10-08	Geneticin 108321-42-2	US $30.00 / KG	1KG	99%	20T	Firsky International Trade (Wuhan) Co., Ltd
2023-09-12	Geneticin 108321-42-2	US $100.00 / bag	1bag	99	5000	Hebei Fengqiang Trading Co., LTD
2023-08-30	Geneticin 108321-42-2	US $0.00 / KG	1KG	99%	50000KG/month	Hebei Mojin Biotechnology Co., Ltd

Geneticin
108321-42-2
US $30.00 / KG
99%
Firsky International Trade (Wuhan) Co., Ltd

Geneticin
108321-42-2
US $100.00 / bag
99
Hebei Fengqiang Trading Co., LTD

Geneticin
108321-42-2
US $0.00 / KG
99%
Hebei Mojin Biotechnology Co., Ltd

Geneticin Spectrum

Geneticin(108321-42-2)MS

108321-42-2(Geneticin)Related Search:

D-Glucosamine sulfate Sodium sulfate sulfate Glucosamine sulfate 4-Aminotetrahydropyran

(R)-2-Aminocyclohenanol G418 TRANS-2-AMINOCYCLOHEXANOL HYDROCHLORIDE 1,3-Diaminocyclohexane TRANS-1,2-CYCLOHEXANEDIOL

PROPYLENE GLYCOL BUTYL ETHER 1-AMINO-3,3-DIETHOXYPROPANE 2-Aminocyclohexanol trans-4-Aminocyclohexanol 3-(CYCLOHEXYLOXY)PROPAN-1-AMINE

3-AMINO-4-METHYL-PENTAN-1-OL 3-Amino-cyclohexanol Cyclohexanol, 2-amino-, (1S,2S)-

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TRYPSIN INHIBITOR SOYBEAN REAGENT GRADE G-418 disulfate,Geneticin Geneticin Disulfate (G418), Antibiotic for Culture Media Use Only Antibiotic G418 Geneticin® 2-[4,6-diamino-3-[3-amino-4,5-dihydroxy-6-(1-hydroxyethyl)oxan-2-yl]oxy-2-hydroxycyclohexyl]oxy-5-methyl-4-(methylamino)oxane-3,5-diol,sulfuric acid G 418 Sulfate, Sterile-Filtered Aqueous Solution, Cell Culture Tested D-Streptamine, O-2-amino-2,7-dideoxy-D-glycero-α-D-gluco-heptopyranosyl-(1→4)-O-[3-deoxy-4-C-methyl-3-(methylamino)-β-L-arabinopyranosyl-(1→6)]-2-deoxy-, sulfate (1:2) Geneticin (G418 Sulfate) ANTIBIOTIC G-418 SULFATE G-418 SULPHATE G418 SULFATE GENETICIN G418 SOLUTION G418 DISULFATE CELL CULTURE TESTED G418 DISULFATE PLANT CELL CULTURE TESTED G418, DISULFATE SALT, BIOTECHNOLOGY PERFORMANCE CERTIFIED GENETICIN DISULFATE CELL CULTURE TESTED GENETICIN SOLUTION CELL CULTURE TESTED GENETICIN DISULFATE G418 SULFATE CELL CULTURE TESTED G 418 Disulfate Aqueous Solution G418 SULFATE (GENETICIN DISULFATE) G 418 Sulfate, cell culture tested G 418 solution disulfate salt G418 sulfateGeneticin®: G418 sulfate(Geneticin)>600U/mg G-418,Geneticin Geneticine disulfate (G 418) G 418 disulfate salt,>=650 U/Mg 620u/Mg Geneticin Sulfate USP G418 disulfate salt, for biocheMistry GeneticinSulfate/G418Sulfate Geneticinedisulfate G418 sulfate, for biochemistry G-418.SulfateSolid g 418disulfate salt solution Geneticin sulfate. Geneticin disulfate saltPotency ≥ 700 ug/mg (anhydrous basis) ) 50mg/ml Solution, Sterile-filtered G 418 disulfate salt, potency: ≥650 μg per mg G-418 disulfate (Geneticin sulfate) G 418 Sulfate, Cell Culture Tested - CAS 108321-42-2 - Calbiochem G 418 Sulfate, Sterile-Filtered Aqueous Solution, Cell Culture Tested - CAS 108321-42-2 - Calbiochem GENETICIN SULFATE;ANTIBIOTIC G-418 SULFATE 50mg/ml G-418 Sulfate Solution, Animal-derived-free G418Disulfate> Male clove extract 418 DisuL G418 DisuL (2R,3S,4R,5R,6S)-5-Amino-6-(((1R,2S,3S,4R,6S)-4,6-diamino-3-(((2R,3R,4R,5R)-3,5-dihydroxy-5-methyl-4-(methylamino)tetrahydro-2H-pyran-2-yl)oxy)-2-hydroxycyclohexyl)oxy)-2-((R)-1-hydroxyethyl)tetrahydro-2H-pyran-3,4-diol bis(sulfate) Geneticin(G 418 disulfate salt) 2-[4,6-diamino-3-[3-amino-4,5-dihydroxy-6-(1-hydroxyethyl)oxan-2-yl]oxy-2-hydroxycyclohexyl]oxy-5-methyl-4-(methylamino)oxane-3,5-diol,sulfuricaci G418 Sulfate (>700U/mg) USPGrade G 418 disulfate salt,≥65 U/mg G-418 Sulphate (Geneticin) extrapure G-418 Sulphate (Geneticin) for tissue culture 17alpha-Epoxypregnenolone