DPNI

DPNI 化学構造式
81295-14-9
CAS番号.
81295-14-9
化学名:
DPNI
别名:
DPNI
英語名:
DPN I
英語别名:
DPN I;DPN I RESTRICTION ENZYME;restriction endonuclease Dpn I;RESTRICTION ENDONUCLEASES DPN I;dpn i from diplococcus pneumoniae;restriction endonuclease dpn I from*diplococcus P;RESTRICTION ENDONUCLEASE DPN I FROM*DIPL OCOCCUS PNE
CBNumber:
CB0256555
化学式:
分子量:
0
MOL File:
Mol file

DPNI 物理性質

貯蔵温度 :
-20°C
外見 :
緩衝グリセロール水溶液

安全性情報

RIDADR  UN3373 - class 6.2 Biological substance, Category B
WGK Germany  3

DPNI 価格 もっと(3)

メーカー 製品番号 製品説明 CAS番号 包装 価格 更新時間 購入
Sigma-Aldrich Japan DPNI-RO from Diplococcus pneumoniae
Dpn I from Diplococcus pneumoniae
81295-14-9 1000UNITS ¥25900 2024-03-01 購入
Sigma-Aldrich Japan DPNI-RO from Diplococcus pneumoniae
Dpn I from Diplococcus pneumoniae
81295-14-9 10742970001 ¥5100 2018-12-25 購入
Sigma-Aldrich Japan DPNI-RO from Diplococcus pneumoniae
Dpn I from Diplococcus pneumoniae
81295-14-9 10742988001 ¥20600 2018-12-25 購入

DPNI 化学特性,用途語,生産方法

使用

In PCR-mediated mutagenesis, DpnI nuclease has been used for the digestion of wild type DNA after PCR.

一般的な説明

Isoschizomers
The enzyme is an isoschizomer to Bsp143 I, Dpn II, Mbo I, Nde II and Sau3A I.
Methylation sensitivity
The enzyme is only inhibited by the occurrence of 5-methylcytosine at the indicated site (*) if no 6-methyladenine is present.
Typical ligation and recutting assay
Dpn I fragments obtained by complete digestion of 1μg pBR322 DNA are ligated for 16 hours at +4°C with 1U T4 DNA Ligase in 10μl buffer that contains 66mM Tris-HCl, 5mM MgCl2, 5mM Dithiothreitol, 1mM ATP, pH 7.5 (at +20°C). The percentages of product that can be ligated and subsequently recut with Dpn I (yielding the typical pattern of pBR322 × Dpn I fragments) are stated under "Lig" and "Rec" in the certificate of analysis.

DPNI 上流と下流の製品情報

原材料

準備製品


DPNI 生産企業

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