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| Product Name: | PFM01 | | Synonyms: | PFM01;(5Z)-5-[(4-Hydroxyphenyl)methylene]-3-(2-methylpropyl)-2-thioxo-4-thiazolidinone;PFM01 >=98% (HPLC);(Z)-5-(4-Hydroxybenzylidene)-3-isobutyl-2-thioxothiazolidin-4-one;Inhibitor,derivative,MRE11,PFM-01,NHEJ,Mirin,DSB,endonuclease,N-alkylated,PFM01,PFM 01,inhibit;4-Thiazolidinone, 5-[(4-hydroxyphenyl)methylene]-3-(2-methylpropyl)-2-thioxo-, (5Z)- | | CAS: | 1558598-41-6 | | MF: | C14H15NO2S2 | | MW: | 293.4 | | EINECS: | | | Product Categories: | | | Mol File: | 1558598-41-6.mol |  |
| | PFM01 Chemical Properties |
| Boiling point | 435.2±55.0 °C(Predicted) | | density | 1.35±0.1 g/cm3(Predicted) | | storage temp. | 2-8°C | | solubility | Soluble in DMSO | | form | powder | | pka | 8.59±0.30(Predicted) | | color | white to beige |
| | PFM01 Usage And Synthesis |
| Biochem/physiol Actions | PFM01 is a cell-permeable N-alkylated Mirin (Sigma Cat. No. 475954) derivative that selectively inhibits against MRE11 endo-, but not exo-, nuclease activity. PFM01 targets MRE11 at a site near the dimer interface, distinct from that occupied by Mirin and PFM39 to allow disruption of the ssDNA-binding groove and selective inhibition against MRE11 endo-, but not exo-, nuclease activity. While both endonuclease and exonuclease activities are required for MRE11-mediated homologous recombination (HR) repair, only FM01 (100 μM), but not the exonuclease inhibitors Mirin (500 μM) and PFM39 (100 μM), rescues G2-phase double-strand break (DSB) repair defect in HR protein BRCA2-deficient HSC62-hTERT fibroblasts following ionizing irradiation (IR) by blocking HR initiation and thereby allowing non-homologous end joining (NHEJ) to proceed. | | storage | Store at -20°C |
| | PFM01 Preparation Products And Raw materials |
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