Hygrolidin
| 中文名称 | Hygrolidin |
|---|---|
| 中文同义词 | 潮内酯 |
| 英文名称 | Hygrolidin |
| 英文同义词 | 1166C;Antibiotic 1166C;Hygrolidin;2-Butenedioic acid (2E)-, mono[(2R,4R,5S,6R)-6-ethyltetrahydro-2-hydroxy-2-[(1S,2R,3S)-2-hydroxy-3-[(2R,3S,4E,6E,9S,10S,11R,12E,14E)-10-hydroxy-3-methoxy-7,9,11,13,15-pentamethyl-16-oxooxacyclohexadeca-4,6,12,14-tetraen-2-yl]-1-methylbutyl]-5-methyl-2H-pyran-4-yl] ester (9CI) |
| CAS号 | 83329-73-1 |
| 分子式 | C38H58O11 |
| 分子量 | 690.86 |
| EINECS号 | |
| 相关类别 | 微生物代谢物;Antibiotic |
| Mol文件 | 83329-73-1.mol |
| 结构式 |  |
Hygrolidin 性质
| 沸点 | 821.1±65.0 °C(Predicted) |
|---|---|
| 密度 | 1.17±0.1 g/cm3(Predicted) |
| 储存条件 | Store at -20°C |
| 溶解度 | 溶于二甲基亚砜 |
| 形态 | 固体 |
| 酸度系数(pKa) | 3.28±0.10(Predicted) |
Hygrolidin (0.1, 1 μg/ml; for 24 h) increases both G1 and S phase populations and decreases M phase population.
Hygrolidin (0.1, 1, 10 μg/ml; for 24 h) decreases the amounts of cdk4, cyclin D, cyclin B and increases the amounts of cyclin E and p21. Hygrolidin-induced p21 preferentially associates with cyclin A-cdk2 complex and inhibits it.
Hygrolidin (0.1, 1 μg/ml; for 24 h) selectively induces p21 in DLD-1 cells at mRNA level, but not in WI-38 fibroblasts.
Hygrolidin, for 3 days, inhibits growth of various cell lines including DLD-1 colon cancer, LNCaP prostate cancer, K562 leukemia cells, LNCaP prostate cancer, EL-4 lymphoma (IC
50
s = 1.0-33 ng/ml, respectively).
Hygrolidin shows growth inhibition on
Trypanosoma cruzi
(IC
50
=1.1 nM),
Trypanosoma brucei brucei
(IC
50
=77 nM), and
Leishmania donovani
(IC
50
=72.5 nM).
Cell Cycle Analysis
| Cell Line: | DLD-1 cells |
| Concentration: | 0.1, 1 μg/ml |
| Incubation Time: | For 24 hours |
| Result: | Increased both G1 and S phase populations and decreased M phase population. |
Western Blot Analysis
| Cell Line: | DLD-1 cells |
| Concentration: | 0.1, 1, 10 μg/ml |
| Incubation Time: | For 24 hours |
| Result: | Decreased the amounts of cdk4, cyclin D, cyclin B and increased the amounts of cyclin E and p21. |
RT-PCR
| Cell Line: | DLD-1 or WI-38 cells |
| Concentration: | 0.1, 1 μg/ml |
| Incubation Time: | For 24 hours |
| Result: | Selectively induced p21 in DLD-1 cells at mRNA level, but not in WI-38 fibroblasts. |
安全信息
| 海关编码 | 29419000 |
|---|