Product Number: CS3409
CAS Number: 3483-12-3
Appearance:White Powder
Molecular formula: C4H10O2S2
Molecular weight:154.25 g/mol
Solubility:0.1 M in H2O, 20℃, complete, colorless
Storage Temperature: 2-8℃
DTT is synthetic. A method of preparation has been described in J. Chem. Soc. 253 (1949).
DTT is an agent for reducing -S-S- to -SH. Redox potential: -0.33 volts at pH 7. DTT acts as a protective agent for -SH groups of other species in solution. In this reaction the DTT is oxidized to the cyclic disulfide, and thereby ensures the reduction of other disulfides in solution. The disulfide reduction is complete in minutes at pH 8. A specific and sensitive assay for disulfide groups is based on determination of resulting monothiols with DTNB.
Applications:
It is used in buffers for isolation of RNA or buffers used for RT-PCR starting with mRNA.
Precautions and Disclaimer:
For Laboratory Use Only. Not for drug, household or other uses.
Preparation Instructions:
The solubility of DTT has been tested by Fluka at 50 mg/ml in water, resulting in a clear colorless solution. It has been described as freely soluble in water, ethanol, acetone, ethylate, chloroform and ether. Solutions oxidize relatively slowly in air. It is recommended to make solutions fresh daily.
关键字: DTT ;二硫苏糖醇;
金克隆生物公司位于北京经济技术开发区,主要从事生物医药试剂的研发和生产,并兼营部分生化试剂和仪器耗材,主营方向有细胞生物学、分子生物学、蛋白与免疫、组织学、微生物培养等相关试剂与耗材。库存现货10000多种,其中我公司自主开发的分子生物学试剂、细胞培养及检测试剂、分子免疫学产品等在质量和价格方面具有较大的市场优势,欢迎广大经销商洽谈合作。