| Identification | More | [Name]
Disodium 4-nitrophenylphosphate | [CAS]
4264-83-9 | [Synonyms]
4-NITROFENYL PHOSPHATE, DISODIUM SALT
4-NITROPHENYL DISODIUM ORTHOPHOSPHATE
4-NITROPHENYL PHOSPHATE, DISODIUM SALT
4-NITROPHENYL PHOSPHATE NA2-SALT
4-NPP
DI-SODIUM 4-NITROPHENYL PHOSPHATE
DISODIUM P-NITROPHENYL PHOSPHATE
P-NITROPHENYL PHOSPHATE DISODIUM
P-NITROPHENYL PHOSPHATE, DISODIUM SALT
P-NITROPHENYL PHOSPHATE LIQUID SUBSTRATE SYSTEM
P-NITROPHENYLPHOSPHORIC ACID DISODIUM SALT
PNPP
PNPP, 2NA
PNP-PHOS DINA
PNPP LIQUID SUBSTRATE SYSTEM
Phosphoricacid,mono(4-nitrophenyl)ester,disodiumsalt
Phosphoricacid,mono(p-nitrophenyl)ester,disodiumsalt
4-Nitrophenyl disodium
4-NITROPHENYL PHOSPHATE DISO-SALT, PGE W . 50 PC., 20 MG/TA
P-NITROPHENYL PHOSPHATE DISODIUM*HEXAHYD RATE CELL | [EINECS(EC#)]
224-246-5 | [Molecular Formula]
C6H4NNa2O6P | [MDL Number]
MFCD00007319 | [Molecular Weight]
263.05 | [MOL File]
4264-83-9.mol |
| Chemical Properties | Back Directory | [Appearance]
White crystalline | [Melting point ]
>300 °C(lit.)
| [storage temp. ]
2-8°C
| [solubility ]
H2O: 1 tablet/3 mL 25 mM, clear, light yellow
| [form ]
tablets
| [color ]
white to pale yellow
| [Stability:]
Stable, but may be light sensitive. Incompatible with strong acids, strong bases, strong oxidizing agents. | [Water Solubility ]
Miscible with water. Immiscible with ethanol and acetone. | [Detection Methods]
HPLC | [BRN ]
5324661 | [InChIKey]
KAKKHKRHCKCAGH-UHFFFAOYSA-L | [CAS DataBase Reference]
4264-83-9(CAS DataBase Reference) | [Storage Precautions]
Light sensitive | [EPA Substance Registry System]
4264-83-9(EPA Substance) |
| Safety Data | Back Directory | [Hazard Codes ]
Xi | [Risk Statements ]
R36/38:Irritating to eyes and skin .
R36/37/38:Irritating to eyes, respiratory system and skin . | [Safety Statements ]
S26:In case of contact with eyes, rinse immediately with plenty of water and seek medical advice .
S36:Wear suitable protective clothing .
S24/25:Avoid contact with skin and eyes .
S23:Do not breathe gas/fumes/vapor/spray (appropriate wording to be specified by the manufacturer) . | [WGK Germany ]
3
| [F ]
10-21 | [TSCA ]
Yes | [HS Code ]
29199000 |
| Hazard Information | Back Directory | [Chemical Properties]
White crystalline | [Uses]
PNPP, Disodium Salt is a colorimetric alkaline phosphatase soluble substrate, p-Nitrophenyl Phosphate (pNPP) is the substrate of choice for use with alkaline phosphatase in Enzyme Linked Immunosorbant Assay (ELISA) procedures. | [General Description]
Excellent substrate for alkaline phosphatase-based ELISA assays. Produces a yellow soluble end product that can be read at 405 nm to 410 nm. Both kinetic and endpoint determinations can be performed. ε max: ≥9600 cm-1 M-1 at 311 nm in 0.4 M Na2CO3. | [Biochem/physiol Actions]
Primary TargetSubstrate for phosphatase | [Purification Methods]
Dissolve it in hot aqueous MeOH, filter and precipitate it by adding Me2CO. Wash the solid with Me2CO and repeat the purification. Aqueous MeOH and Et2O can also be used as solvents. The white fibrous crystals contain less than 1% of free 4-nitrophenol [assay: Axelrod J Biol Chem 167 57 1947]. [Beilstein 6 IV 1327.] |
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