| Identification | More | [Name]
PIPES | [CAS]
5625-37-6 | [Synonyms]
1,4-PIPERAZINEBIS(ETHANESULFONIC ACID)
1,4-PIPERAZINEDIETHANESULFONIC ACID
LABOTEST-BB LT00138111
PIPERAZINE-1,4-BIS(2-ETHANESULFONIC ACID)
PIPERAZINE-N,N'-BIS(2-ETHANESULFONIC ACID)
PIPERAZINE-NN'-BIS-2-ETHANESULPHONIC ACID
PIPES
TIMTEC-BB SBB008973
1,4-Piperazinebis(ethanesulphonic acid)
2,2-(piperazine-1,4-diyl)bis(ethanesulphonic) acid
PIPES Piperazine-N,N-bis(2-ethanesulfonic acid)
PIPES, Free Acid, MB Grade (1.12030)
PIPES, Free Acid, ULTROL Grade
PIPES, Molecular Biology Grade Piperazine-N,N-bis(2-ethanesulfonic acid), Molecular Biology Grade
Piperazine-1,4-bis(ethanesulfonic acid)
PIPERAZINE-1 4-BIS(2-ETHANESULFONIC ACI&
PIPES FREE ACID SIGMAULTRA
PIPES FREE ACID, BIOTECHNOLOGY PERFORMAN CE CERTIFIE
PIPES FREE ACID
PIPERAZINE-1,4-BIS(2-ETHANESULFONIC ACID ), F. MOL.BIOLOGY | [EINECS(EC#)]
227-057-6 | [Molecular Formula]
C8H18N2O6S2 | [MDL Number]
MFCD00006159 | [Molecular Weight]
302.37 | [MOL File]
5625-37-6.mol |
| Chemical Properties | Back Directory | [Appearance]
White/clear crystalline powder | [Melting point ]
>300 °C (lit.) | [density ]
1.4983 (rough estimate) | [refractive index ]
1.6300 (estimate) | [storage temp. ]
Store at RT. | [solubility ]
0.1 M NaOH: 0.25 g/mL, clear, colorless
| [form ]
Micro-Crystalline Powder | [color ]
White | [PH Range]
6.1 - 7.5 | [pka]
6.8(at 25℃) | [Water Solubility ]
Soluble in water. | [λmax]
λ: 260 nm Amax: 0.055
λ: 280 nm Amax: 0.040 | [Detection Methods]
T | [Merck ]
14,7479 | [BRN ]
817713 | [InChIKey]
XWGRYGKFRRLUQQ-UHFFFAOYSA-N | [LogP]
-2.7 at 20℃ | [CAS DataBase Reference]
5625-37-6(CAS DataBase Reference) | [EPA Substance Registry System]
1,4-Piperazinediethanesulfonic acid (5625-37-6) |
| Safety Data | Back Directory | [Hazard Codes ]
Xi | [Risk Statements ]
R36/37/38:Irritating to eyes, respiratory system and skin . | [Safety Statements ]
S22:Do not breathe dust .
S24/25:Avoid contact with skin and eyes .
S37/39:Wear suitable gloves and eye/face protection .
S26:In case of contact with eyes, rinse immediately with plenty of water and seek medical advice . | [WGK Germany ]
3
| [TSCA ]
Yes | [HS Code ]
29335995 |
| Questions And Answer | Back Directory | [description]
PIPES free acid, also known as 1,4-piperazinediethanesulfonic acid, is a Good's buffer notable for having a pKa value similar to ordinary physiological pH. As such, PIPES free acid is frequently used as a buffer in biochemical research.
PIPES is a zwitterionic, piperazinic buffer that is useful for a pH range of 6.1 – 7.5. PIPES lacks the ability to form a significant complex with most metal ions and is recommended for use as a non-coordinating buffer in solutions with metal ions. PIPES has wide variety of applications and is commonly used in cell culture media, in protein crystallization, as a running buffer in gel electrophoresis, and as an eluent in isoelectric focusing and chromatography. This buffer is capable of forming radicals and is therefore not suitable for redox reactions. It is suitable for use in the bicinchoninic acid (BCA) assay. Solubility of PIPES increases when the free acid is converted to the sodium salt.
Buffers are made by adding base solution to PIPES free acid and titrating to the desired pH. | [Uses]
Piperazine-N, N'-bis- (2-ethanesulphonic acid) ultra -pure >99.5%) is an important chemical intermedia in all kinds of chemical fields. 1,4-Piperazinediethanesulfonic acid, piperazine-N, N’-bis- (2-ethanesulfonic acid) (PIPES) is also one of the zwitterion buffers and the most appropriate buffer for the biological experiments using an intracellular solution since the Pk2 of Pipes is 6.8 at 20 degrees Celsius.
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| Hazard Information | Back Directory | [Chemical Properties]
White/clear crystalline powder | [Application]
Glutaraldehyde fixation of plant and animal tissue samples can cause loss of lipid, leading to apparent morphological changes. Lipid loss and artifacts were minimized when PIPES was used to buffer the glutaraldehyde fixative.
Alkaline phosphatase activity is lost selectively from certain rat hepatocyte organelles when fixed for ultracytochemistry with cacodylate-buffered glutaraldehyde. When PIPES was used as buffer, retention of activity was 60% greater.
Fixation of fungal zoospores for fluorescence microscopy and electron microscopy was optimal with a combination of glutaraldehyde and formaldehyde in PIPES buffer. | [Definition]
ChEBI: 2,2'-piperazine-1,4-diylbisethanesulfonic acid is a Good's buffer substance, pKa = 6.8 at 20 ℃. It is a conjugate acid of a 2,2'-piperazine-1,4-diylbisethanesulfonate. | [General Description]
PIPES is a member of the ethanesulfonic acid buffer series, first introduced by Good et al., developed to meet certain criteria: midrange pKa, maximum water solubility and minimum solubility in all other solvents, minimal salt effects, minimal change in pKa with temperature, chemically and enzymatically stable, minimal absorption in visible or UV spectral range and easily synthesized. Since its pKa at 37 °C is near physiological pH, PIPES has applications in cell culture work. | [Flammability and Explosibility]
Notclassified | [Purification Methods]
Purify PIPES from boiling water (maximum solubility is about 1g/L) or as described for ADA [N-(2-acetamido)iminodiacetic acid above]. [Good et al. Biochemistry 5 469 1966, Beilstein 23/12 V 380.] |
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