4-(2-Hydroxyethyl)piperazin-1-ylethansulfonsure
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- CAS-Nr.
- 7365-45-9
- Bezeichnung:
- 4-(2-Hydroxyethyl)piperazin-1-ylethansulfonsure
- Englisch Name:
- HEPES
- Synonyma:
- HEPES BUFFER;4-(2-HYDROXYETHYL)-1-PIPERAZINEETHANESULFONIC ACID;hepes solution;HYDROXYETHYLPIPERAZINE ETHANE SULFONIC ACID;Biological Buffer;HEPES buffer Solution;4-(2-HYDROXYETHYL)PIPERAZINE-1-ETHANESULFONIC ACID;4-(2-hydroxyethyl)piperazin-1-ylethanesulphonic acid;2-[4-(2-HYDROXYETHYL)-1-PIPERAZINYL]ETHANESULFONIC ACID;EPES
- CBNumber:
- CB5408557
- Summenformel:
- C8H18N2O4S
- Molgewicht:
- 238.3
- MOL-Datei:
- 7365-45-9.mol
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4-(2-Hydroxyethyl)piperazin-1-ylethansulfonsure Eigenschaften
- Schmelzpunkt:
- 234-238 °C
- Siedepunkt:
- 408℃[at 101 325 Pa]
- Dichte
- 1.07 g/mL at 20 °C
- Dampfdruck
- 0Pa at 25℃
- Brechungsindex
- n20/D 1.339
- storage temp.
- 2-8°C
- Löslichkeit
- H2O: 1 M at 20 °C, clear, colorless
- Aggregatzustand
- powder
- Farbe
- White
- PH
- 5.0-6.5 (1Maqueous solution)
- pka
- 7.5(at 25℃)
- Säure-Base-Indikators(pH-Indikatoren)
- 7.0 - 7.6
- Wasserlöslichkeit
- Soluble
- maximale Wellenlänge (λmax)
- λ: 260 nm Amax: 0.06
λ: 280 nm Amax: 0.04
- Merck
- 14,4654
- BRN
- 883043
- Stabilität:
- Stable. Combustible. Incompatible with strong oxidizing agents. Protect from moisture.
- InChIKey
- JKMHFZQWWAIEOD-UHFFFAOYSA-N
- LogP
- -3.85 at 20℃
- CAS Datenbank
- 7365-45-9(CAS DataBase Reference)
- EPA chemische Informationen
- 1-Piperazineethanesulfonic acid, 4-(2-hydroxyethyl)- (7365-45-9)
Sicherheit
- Risiko- und Sicherheitserklärung
- Gefahreninformationscode (GHS)
| Kennzeichnung gefährlicher |
Xi |
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| R-Sätze: |
36/37/38 |
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| S-Sätze: |
24/25-22-36-26 |
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| WGK Germany |
1 |
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| RTECS-Nr. |
TL6809000 |
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| TSCA |
Yes |
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| HS Code |
29335995 |
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| Bildanzeige (GHS) |
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| Alarmwort |
Warnung |
| Gefahrenhinweise |
| Code |
Gefahrenhinweise |
Gefahrenklasse |
Abteilung |
Alarmwort |
Symbol |
P-Code |
| H302 |
Gesundheitsschädlich bei Verschlucken. |
Akute Toxizität oral |
Kategorie 4 |
Warnung |
![GHS hazard pictograms]() src="/GHS07.jpg" width="20" height="20" /> |
P264, P270, P301+P312, P330, P501 |
| H315 |
Verursacht Hautreizungen. |
Hautreizung |
Kategorie 2 |
Warnung |
![GHS hazard pictograms]() src="/GHS07.jpg" width="20" height="20" /> |
P264, P280, P302+P352, P321,P332+P313, P362 |
| H319 |
Verursacht schwere Augenreizung. |
Schwere Augenreizung |
Kategorie 2 |
Warnung |
![GHS hazard pictograms]() src="/GHS07.jpg" width="20" height="20" /> |
P264, P280, P305+P351+P338,P337+P313P |
| H335 |
Kann die Atemwege reizen. |
Spezifische Zielorgan-Toxizität (einmalige Exposition) |
Kategorie 3 (Atemwegsreizung) |
Warnung |
![GHS hazard pictograms]() src="/GHS07.jpg" width="20" height="20" /> |
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| Sicherheit |
| P261 |
Einatmen von Staub vermeiden. |
| P271 |
Nur im Freien oder in gut belüfteten Räumen verwenden. |
| P280 |
Schutzhandschuhe/Schutzkleidung/Augenschutz tragen. |
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4-(2-Hydroxyethyl)piperazin-1-ylethansulfonsure Chemische Eigenschaften,Einsatz,Produktion Methoden
R-Sätze Betriebsanweisung:
R36/37/38:Reizt die Augen, die Atmungsorgane und die Haut.
S-Sätze Betriebsanweisung:
S24/25:Berührung mit den Augen und der Haut vermeiden.
S22:Staub nicht einatmen.
S36:DE: Bei der Arbeit geeignete Schutzkleidung tragen.
S26:Bei Berührung mit den Augen sofort gründlich mit Wasser abspülen und Arzt konsultieren.
Beschreibung
HEPES has been described as one of the best all-purpose buffers available for biological research. At biological pH, the molecule is zwitterionic, and is effective as a buffer at pH 6.8 to 8.2 (pKa 7.55). It is typically used in cell culture at concentration between 5mM to 30 mM. HEPES has been used in a wide variety of applications, including tissue culture. It is commonly used to buffer cell culture media in air. HEPES finds its usage in in vitro experiments on Mg.
Chemische Eigenschaften
White crystalline powder
Verwenden
Non-toxic to cell. It is used as a hydrogen ion buffer, which can control the constant pH range for long term. The concentration is 10-50mmol/L. Generally, 20mmol/LHEPES in nutrient solution can get buffer ability.
Definition
ChEBI: A HEPES that is ethanesulfonate substituted by a 4-(2-hydroxyethyl)piperazin-4-ium-1-yl group at position 2.
Allgemeine Beschreibung
HEPES buffered saline is a tissue culture buffer, which is used to maintain the pH of cell culture. It is water soluble and atmosphere independent. HEPES destroys magnesium in sodium chloride solutions.
Biologische Aktivität
HEPES is a multi purpose HEPES buffer used in cell culture and other biological research. Working pH range in aqueous solution: 6.8 - 8.2. Does not form complexes with metal ions. Used in cell culture media.
läuterung methode
Crystallise the acid from hot EtOH and water. It is a useful buffer. [Beilstein 23 V 376.]
4-(2-Hydroxyethyl)piperazin-1-ylethansulfonsure Upstream-Materialien And Downstream Produkte
Upstream-Materialien
Downstream Produkte
4-(2-Hydroxyethyl)piperazin-1-ylethansulfonsure Anbieter Lieferant Produzent Hersteller Vertrieb Händler.
Global( 706)Lieferanten
7365-45-9(4-(2-Hydroxyethyl)piperazin-1-ylethansulfonsure)Verwandte Suche:
- HEPES 2-[4-(2-HYDROXYETHYL)-1-PIPERAZINYL]ETHANESULFONIC ACID MOLECULAR BIOLOGY GRADE
- HEPES 2-[4-(2-HYDROXYETHYL)-1-PIPERAZINYL]ETHANESULFONIC ACID 99+%
- N-(2-HYDROXYETHYL)-PIPERAZINE-N'-2-ETHANESULFONIC ACID(4-(2-HYDROXYETHYL)PIPERAZINE-1-ETHANESULFONIC ACID(HEPES-ACID))
- Hepes,FreeAcid(N-[2-Hydroxyethyl]Piperaz
- 1-HeptaneSulfonicAcidSod.SaltGr
- N-(2-Hydroxyethyl)Piperazine-N'-(3-EthanesulfonicAcid)
- N-(2-Hydroxyethyl)Piperazine-N'-(2-EthanesulfonicAcid)(Hepes)
- Hepes,LowSodium,FreeAcid
- HEPES free acid, DNase, Rnase and Protease free, for molecular biology, 99+%
- Hydroxyethyl)-1-piperazineethyanesulfonic acid, 4-(2-
- 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid(HEPES)
- 2-(4-(2-Hydroxyethyl)piperazin-1-yl)ethanesulfonic acid
- 4-(2-hydroxyerhyl)piperazine-1-erhaesulfonic acid
- HEPES, for biochemistry, 99%
- 4-(2-Hydroxyethyl)-1-Piperazin
- Hydroxyethyl)piperazine-N'-2-ethane sulfonic acid
- 1-Piperazineethanesulfonic acid, 4-(2-hydroxyethyl)-
- HEPES,BIOLOGICALBUFFER
- HEPES,BIOLOGICALBUFFER,ULTRAPURE
- N-(2-hydroxymethyl)piperazine-N｀-(2-ethanesulfonic acid)(HEPES)
- N-2-HYDROXYETHYLPIPERAZINE-N-2-ETHANSULFONICACID(HEPES)
- 4-(2-Hydroxyethyl)piperazin-1-ylethansulfonsure
- 2-[4-(2-Hydroxyethyl)-1-piperazine]ethanesulfonic acid HEPES
- HEPES / 4-(2-HYDROXYETHYL)-1-PIPERAZINEETHANESULPHONIC ACID
- HEPES (4-(2-HYDROXYETHYL)PIPERAZINE-1-ETHANESULFONIC ACID)
- HEPES ULTRAPURE
- N-2-HYDROXYETHYLPIPERAZINE-N''-2-ETHANE SULPHONIC ACID >99.5%
- N-[2-HYDROXYERHYL]PIPERAZINE-N''-ETHANESULFONICACID(HEPES)
- HEPES free acid ultrapure
- N-2-Hydroxyethylpiperazine-N'-2-ethanesulphonic acid, low sodium >99.5%
- 2-[4-(2-Hydroxyethyl)-1-piperazinyl]ethanesulfonic Acid [Good's buffer component for biological research]
- HEPES(N-[2-Hydroxyethyl]piperazine-N'-[Ethanesulfonic acid]),Free Acid
- N'-2-N-2-hydroxyethyl piperazineethanesulfonic acid
- HEPES, >=99.5% (titration)
- HEPES SynonyMs 4-(2-Hydroxyethyl)-1-piperazineethanesulfonic acid
- HEPES Buffer Solution (1M)
- HEPES,4-(2-hydroxyerhyl)piperazine-1-erhanesulfonic acid
- HEPES Vetec(TM) reagent grade, 99.5%
- 4-CHLORO-1-NAPHTHOL ULTRA PURE GRADE
- POTASSIUM HYDROXIDE 0,05 MOL DUTY PAID
- POTASSIUM HYDROXIDE 0,5MOL/L DUTY PAID
- POTASSIUM HYDROXIDE 1 MOL/L DUTY PAID
- SELENIUM STAND
- MALATE DEHYDROGENASE ULTRA PURE GRADE
- PHOSPHOTUNGSTIC ACID HIGH PURITY GRADE
- 4-(2-hydroxyethyl)-1-piperazineethanesulfonicaci
- hepesfreeacid
- 2-[4-(2-HYDROXYETHYL)-1-PIPERAZINE]ETHANESULFONIC ACID
- LABOTEST-BB LT00138109
- LABOTEST-BB LT00233156
- HEPES
- HEPES-ACID
- HEPES BUFFERED SALINE
- HEPES BUFFER PH 7.5
- 4-(2-Hydroxyethyl)-1-piperazineethanesulphonic acid
- hepes buffer (1M) aseptically filled*endotoxin te
- hepes free acid molecular biology*reagent
- HEPES N-(2-Hydroxyethyl)piperazine-N-(2-ethanesulfonic acid)
