甲异靛
| 中文名称 | 甲异靛 |
|---|---|
| 中文同义词 | N-甲基-(3,3'-双-二氢吲哚)-2,2'-二酮;甲异靛;异甲靛;甲异靛, >96%;1-甲基-[3,3'-联吲哚基] -2,2'-二酮;1-甲基-[3,3'-联吲哚啉亚基] -2,2'-二酮;(E)-1-甲基-[3,3-联亚二氢吲哚基]-2,2-二酮;1-甲基-[3,3'-联吲哚啉亚基] -2,2'-二酮 |
| 英文名称 | Meisoindigo |
| 英文同义词 | 1-METHYL-3,3'-BIINDOLE-2,2'(1H,1'H)-DIONE;3-(1,2-dihydro-2-oxo-3h-indol-3-ylidene)-1,3-dihydro-1-methyl-2h-indol-2-one;MEISOINDIGO;N-methylisoindigotin;Dian III;Methylisoindigotin;Natura-α;Meisoindigo 3-(1,2-Dihydro-2-oxo-3H-indol-3-ylidene)-1,3-dihydro-1-methyl-2H-indol-2-one |
| CAS号 | 97207-47-1 |
| 分子式 | C17H12N2O2 |
| 分子量 | 276.29 |
| EINECS号 | 200-256-5 |
| 相关类别 | 小分子抑制剂;小分子抑制剂，天然产物;医药化工中间体;标准品;中药对照品;Inhibitors |
| Mol文件 | 97207-47-1.mol |
| 结构式 |  |
甲异靛 性质
| 熔点 | 236-237°C |
|---|---|
| 沸点 | 482.0±45.0 °C(Predicted) |
| 密度 | 1.381±0.06 g/cm3(Predicted) |
| 储存条件 | Sealed in dry,Room Temperature |
| 溶解度 | 不溶于水；不溶于乙醇；超声波检测下 DMSO 中≥25.85 mg/mL |
| 形态 | 粉末晶体 |
| 酸度系数(pKa) | 11.62±0.20(Predicted) |
| 颜色 | 橙色到棕色到深红色 |
Meisoindigo (Dian III; 5-20 μM; for 24 hours) inhibits growth of the AML cell lines.
Meisoindigo (10 μM; for 24 hours) induces apoptosis of acute myeloid leukemia.
Meisoindigo (5-10 μM; for 24 hours) causes cell-cycle arrest.
Meisoindigo (5-10 μM; for 24 hours) increases the cleaved caspase-3 and pro-apoptotic Bak, and decreases Bcl-2 and Bcl-xL levels in HL-60 cells.
Meisoindigo (10, 30, 50, 100, 150 μM; 24 hours) interdicts LPS-induced (1 μg/mL) NLRP3 inflammasome activation and M1/M2 polarization through down-regulation of TLR4 pathways after OGD/R in HT-22 and BV2 cells.
Cell Viability Assay
| Cell Line: | HL-60, NB4, U937 leukemic cell lines |
| Concentration: | 5, 10, 15, 20 μM |
| Incubation Time: | For 24 hours |
| Result: | Inhibited growth of the AML cell lines in a dose- and time-dependent manner. |
Apoptosis Analysis
| Cell Line: | HL-60 cells |
| Concentration: | 10 μM |
| Incubation Time: | For 24 hours |
| Result: | Induced apoptosis of acute myeloid leukemia. |
Cell Cycle Analysis
| Cell Line: | HL-60 cells |
| Concentration: | 5, 10 μM |
| Incubation Time: | For 24 hours |
| Result: | Caused cell-cycle arrest, with more cells in sub-G1 and G0/G1 phases and fewer cells in the S phase, in a dose-dependent manner. |
Western Blot Analysis
| Cell Line: | HL-60 cells |
| Concentration: | 5, 10 μM |
| Incubation Time: | For 24 hours |
| Result: | Increased the cleaved caspase-3 and pro-apoptotic Bak, and decreased Bcl-2 and Bcl-xL levels in HL-60 cells. |
Meisoindigo (Dian III; 50-150 mg/kg; IP; daily; for 14 days) has anti-leukemic activity in vivo.
Meisoindigo (2, 4, 8, 12 mg/kg; IP; before MCAO and 2 h after reperfusion) significantly reduces infarct volume, ameliorates neurological deficits 3 days after middle cerebral artery occlusion (MCAO) in Wild-type C57BL/6J mice (25-30 g). Meisoindigo reduces edema and lowers AQP4 expression in the brain.
| Animal Model: | NOD/SCID mice, 6-8 weeks old, with HL-60 leukemic cells |
| Dosage: | 50, 100, 150 mg/kg |
| Administration: | IP; daily; for 14 days |
| Result: | Had anti-leukemic activity in vivo. |
安全信息
| 海关编码 | 2933.79.1500 |
|---|
| 更新日期 | 产品编号 | 产品名称 | CAS号 | 包装 | 价格 |
|---|---|---|---|---|---|
| 2024/04/30 | M3082 | 甲异靛 Meisoindigo | 97207-47-1 | 25mg | 590元 |
| 2024/04/30 | HY-13680 | 甲异靛 Meisoindigo | 97207-47-1 | 5mg | 800元 |