Amonafide

价格	￥977.75
包装	1EA

最小起订量	1EA
发货地	其他
更新日期	2020-01-12

产品详情

英文名称:Amonafide	CAS:69408-81-7
规格: 10mg/10mM/1mL/50mg/100mg	货号: S1367

Selleck Chemicals美国品牌，中国库存现货，Topoisomerase抑制剂，CAS#69408-81-7。
更多详情请访问中国唯一官方网站： http://www.selleck.cn/products/Amonafide.html

生物活性

产品描述	Amonafide (NSC-308847)通过topoisomerase II（拓扑异构酶II）介导导致蛋白相关的DNA链断裂，但不会产生拓扑异构酶I介导的DNA裂解。Phase 3。
靶点	Topoisomerase II
IC50
体外研究	Through a topoisomerase II-mediated reaction, Amonafide treatment produces DNA single-strand breaks (SSB), double-strand breaks (DSB), and DNA-protein cross-links in human myeloid leukemia cells. Amonafide treatment inhibits conlony formation of the leukemic cell lines and the normal human bone marrow GM-CFC in a dose-dependent manner. Amonafide does not produce topoisomerase I-mediated DNA cleavage even at 100 μM. The m-AMSA-resistant line is less than 2-fold resistant to Amonafide [1] Amonafide interferes with the DNA breakage-reunion activity of mammalian DNA topoisomerase II resulting in DNA cleavage stimulation. [2] Compared with those of other antitumor drugs, Amonafide-stimulated cleavage intensity patterns are markedly different. Amonafide highly prefers a cytosine, and excludes guanines and thymines instead, at position -1, with lower preference for an adenine at position +1. [3] Topoisomerase II-mediated DNA cleavage induced by Amonafide is affected only slightly (less than 3-fold) by 1 mM ATP, suggeting that Amonafide is an ATP-insensitive topoisomerase II inhibitor in contrast to doxorubicin, etoposide, and mitoxantrone. [4] Amonafide significantly inhibits the growth of HT-29, HeLa, and PC3 cells with IC50 of 4.67 μM, 2.73 μM, and 6.38 μM, respectively. [5] Amonafide is unaffected by P-glycoprotein-mediated efflux, unlike those of the classical topoisomerase II inhibitors (daunorubicin, doxorubicin, idarubicin, etoposide, and mitoxantrone). [6]
体内研究
临床实验	A Phase I/II study of Amonafide in men with androgen-independent prostate cancer has been completed.
特征

细胞试验:
[5]

细胞系	HT-29, HeLa, and PC3
浓度	Dissolved in DMSO, final concentrations ~10 μM
处理时间	72 hours
方法	All cell lines are in the logarithmic phase of growth when the assay of 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) is carried out. Cells are harvested and seeded into 96-well tissue culture plates at a density of 2.5 × 103 cells/well in 150 μL aliquots of medium. The concentrations tested are serial dilutions of a stock solution (10 μM in DMSO) with phosphate-buffered saline (PBS) and are added 24 hours later. The assay is ended after 72 hours of Amonafide exposure and PBS is used as a negative control. After 72 hours treatment, cells are washed twice with PBS, and then 50 μL/well of MTT reagent (1 mg/mL in PBS) together with 150 μL/well of prewarmed medium are added. The plates are returned to the incubator for 4 hours. Subsequently, DMSO is added as solvent. Absorbance is determined at 570 nm with a Microplate reader. All experiments are performed at least three times, and the average of the percentage absorbance is plotted against concentration. Then, the concentration of Amonafide required to inhibit 50% of cell growth (IC50) is calculated for Amonafide.

温馨提示：不可用于临床治疗。

关键字： Amonafide;化学试剂

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产品名称	价格		公司名称	报价日期
5-氨基-2-(2-(二甲基氨基)乙基)-1H-苯并[de]异喹啉-1,3(2H)-二酮	询价	VIP4年	河南威梯希化工科技有限公司	2024-06-04
aladdin 阿拉丁 A126932 AS1413, 氨萘非特(Amonafide) 69408-81-7 ≥98%	￥338.90	VIP12年	上海阿拉丁生化科技股份有限公司	2024-06-03
aladdin 阿拉丁 A408632 Amonafide 69408-81-7 10mM in DMSO	￥451.90	VIP1年	上海阿拉丁生化科技股份有限公司	2024-06-03
氨萘非特	询价	VIP5年	陕西缔都医药化工有限公司	2024-06-03
Amonafide	￥1600		上海超研生物科技有限公司	2024-05-30
Amonafide (AS1413)	￥900	VIP10年	南京百鑫德诺生物科技有限公司	2024-05-21
Amonafide	￥875		武汉科昊佳生物科技有限公司	2020-01-13
Amonafide	￥700		上海伟寰生物科技有限公司	2020-01-13

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