N-乙酰基神经鞘氨醇
| 中文名称 | N-乙酰基神经鞘氨醇 |
|---|---|
| 中文同义词 | N-乙酰基神经鞘氨醇;N-乙酰基-D-神经鞘氨醇;C2 神经酰胺;N-[(1S,2R,3E)-2-羟基-1-(羟甲基)-3-庚烯-1-基]-乙酰胺;N-乙酰鞘氨醇;化合物 T22644;N-乙酰基-D-鞘氨醇 |
| 英文名称 | C2 CERAMIDE |
| 英文同义词 | N-ACETYL-D-ERYTHRO-SPHINGOSINE;N-ACETYLSPHINGOSINE, D-ERYTHRO;C14 SPHINGOID BASE;C2:0 CERAMIDE;C2-D-ERYTHRO-CERAMIDE;C2 CERAMIDE;C2 CERAMIDE OF D-ERYTHRO-C14-SPHINGOSINE;CERAMIDE C2 |
| CAS号 | 3102-57-6 |
| 分子式 | C20H39NO3 |
| 分子量 | 341.53 |
| EINECS号 | |
| 相关类别 | 生化试剂;小分子;Mixed Fatty Acids;Activators;Fatty Acid Derivatives & Lipids;Glycerols;Inhibitors;Protein Kinase Inhibitors and Activators;Lipid signaling |
| Mol文件 | 3102-57-6.mol |
| 结构式 |  |
N-乙酰基神经鞘氨醇 性质
| 熔点 | 93-96 °C |
|---|---|
| 沸点 | 477.13°C (rough estimate) |
| 密度 | 1.0283 (rough estimate) |
| 折射率 | 1.5614 (estimate) |
| 储存条件 | −20°C |
| 溶解度 | 可溶于DMSO |
| 酸度系数(pKa) | 13.50±0.20(Predicted) |
| 形态 | 粉末 |
| 颜色 | 白色 |
| 稳定性 | 湿气敏感 |
Protein phosphatase 1
PP2A
Ceramide-activated protein phosphatase (CAPP)
Apoptosis
Mitochondrial respiratory chain complex III
C2 Ceramide (5 nM-200 µM; 24 hours; primary mouse osteoblasts) treatment (≤500 nM) promots osteoblast viability, whilst concentrations ≥2 µM significantly reduces osteoblast viability in a dose- and time-dependent manner.
C2 Ceramide increases cytoplasmic histone-associated DNA fragments by 5.7- and 11.2-fold at 50 µM and 100 µM C2 Ceramide concentrations respectively in osteoblasts. At these higher concentrations, C2 Ceramide is a potent inducer of apoptosis in osteoblasts.
C2 Ceramide up-regulates mRNA expression of angiogenic genes in human dental pulp cells (HDPCs) and increases the migration and capillary tube formation of endothelial cells, whereas PP1 small interfering RNA shows opposite effects. Human dental pulp cells (HDPCs) increases levels of bone morphogenetic protein 2, phosphorylation of Smad 1/5/8, and mRNA expression of runt-related transcription factor 2 and osterix.
Cell Viability Assay
| Cell Line: | Primary mouse osteoblasts |
| Concentration: | 5 nM-200 µM |
| Incubation Time: | 24 hours |
| Result: | Murine osteoblasts demonstrated a dose-dependent increase in their survival rate when exposed to low concentrations of 5-500 n M. Increasing concentrations of 20-200µM caused a dose-dependent decrease in mitochondrial succinate dehydrogenase activity and osteoblast survival. |
The PP1 activator C2 Ceramide increases alkaline phosphatase activity, mineralizes nodule formation, and mRNA expression of dentin matrix protein 1 and dentin sialophosphoprotein. In contrast, knockdown by PP1 small interfering RNA inhibits odontoblastic differentiation.
安全信息
| 安全说明 | 22-24/25 |
|---|---|
| WGK Germany | 3 |
| 海关编码 | 29241990 |
| 提供商 | 语言 |
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